Asian Journal of Research in Chemistry

R. V. Rele ¹, S. P. Patil ¹

Affiliations
1 D.G. Ruparel College, Matunga, Mumbai-400016, IN

Abstract

A simple, rapid sensitive and precise UV spectrophotometric method has been developed for the estimation of azelnidipine from bulk drug and pharmaceutical formulation. In this method azelnidipine showed maximum absorbance at about 254 nm in methanol. Beer's law was followed in the concentration range of 1 to 20 μg/ml. Regression equation was found to be y=0.0478 x+0.0013 and coefficient of correlation was 0.9999. The proposed method is sensitive, accurate, reproducible and useful for the estimation of azelnidipine from bulk drug and pharmaceutical formulation.

Keywords

Azelnidipine UV-Spectrophotometer Methanol.

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R. V. Rele ¹, C. B. Warkar ¹

Affiliations
1 D. G. Ruparel College, Matunga, Mumbai-400016, IN

Abstract

A gradient reverse phase HPLC method was developed and validated for simultaneous estimation of ciprofloxacin hydrochloride and dexamethasone in pharmaceutical dosage form i.e. ophthalmic solution using Nucleosil C18 (15 cm×4.6 mm, 5 μm) column with a flow rate of 1.2 ml/minute and detection of 265 nm. The mobile phase A consisted of 50 milli molar citric acid and potassium phosphate buffer. The mobile phase B consisted of 100% v/v acetonitrile. The method was validated for system suitability, linearity, accuracy and precision. The system suitability parameters like resolution, theoretical plates and tailing factor were evaluated. The linear range for ciprofloxacin was 3 μg/ml-55 μg/ml and for dexamethasone was 1 μg/ml-16 μg/ml. The method has been successfully used to analyze pharmaceutical dosage form i.e. ophthalmic solution containing ciprofloxacin hydrochloride and dexamethasone with good recoveries.

Keywords

Ciprofloxacin Hydrochloride, Dexamethasone, Pharmaceutical Dosage Form.

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